Research of The Black Seed

Traditionally used for the promotion of good health and for many ailments, including:

  • Fever
  • Immune system
  • Common cold
  • Asthma
  • Rheumatism
  • Immune disorders
  • Metabolic disorders
  • Microbial infections
  • Intestinal worms
  • Cardiovascular complaints
  • Headaches

The traditional uses for Black seed have been the foundation for many of the studies so far made and have clarified its popularity as a natural remedy for so many centuries in so many lands.

Anti-Inflammatory and Anti-Rheumatic

In February 1995, doctors at King’s College London, U.K. (1) tested Black seed oil’s use for rheumatism and inflammatory diseases.

They concluded that their studies supported the traditional use of Nigella sativa as a treatment for rheumatism and related inflammatory diseases.

In 1960, Professor El-Dakhakny reported that black seed oil has an anti-inflammatory effect and that it could be useful for relieving the effects of arthritis.

In 2002, at the Alexandria Medical Faculty, Alexandria, Egypt he also studied the effectiveness of nigellone and thymoquinone whereby his research partly explained the mode of action of Black seed’s volatile oils in ameliorating inflammatory diseases.

In 2001, doctors at Aga Khan University Medical College, Karachi, Pakistan, investigated the uses of the blessed seed for its bronchodilator and spasmolytic qualities.

Their studies concluded that black seed’s usefulness for diarrhea and asthma in traditional medicine appeared to be based on a sound mechanistic background.

Immune System Support


As a natural remedy people take black seed or oil as a promoter of good health and for the prevention of common cold and asthma.

In 1986, Drs El-Kadi and Kandil at I.I.M.E.R. Panama City, Florida, investigated the effect of the blessed seed on the immune system and studied the effect of 1g taken twice daily in human volunteers.

They concluded, “These findings may be of great practical significance since a natural immune enhancer like the black seed could play an important role in the treatment of cancer, AIDS, and other disease conditions associated with immune deficiency states.”

These results were confirmed by a study published in the Saudi Pharmaceutical Journal in 1993 by Dr Basil Ali and his colleagues from the College of Medicine at King Faisal University.

Five Antibiotics Tested

In 1997 researchers at the Department of Pharmacy, University of Dhaka, Bangladesh, conducting a study in which the antibacterial activity of the volatile oil of black seed was compared with five antibiotics:

  • ampicillin
  • tetracycline
  • cotrimoxazole
  • gentamicin
  • nalidixic acid

The oil proved to be more effective against many strains of bacteria, including those known to be highly resistant to drugs.

V. cholera, E. coli (a common infectious agent found in undercooked meats), and all strains of Shigella spp., except Shigella dysentriae.

Most strains of Shigella have been shown to rapidly become resistant to commonly used antibiotics and chemotherapeutic agents.


Dr. Haq in research on human volunteers at the Department of Biological and Medical Research Center in Riyadh, Saudi Arabia showed that black seed enhanced the ratio between helper T-cells and suppresser T-cells by 55% with a 30% average enhancement of the natural killer cell activity.

These results are effective enough to consider the oil being used in the treatment of AIDS and the findings of this paper caught the attention of the Archives of AIDS where they were duly published.


research2In 2001 at King Faisal University, Saudi Arabia, Dr Al-Ghamdi investigated black seed’s anti-inflammatory, analgesic and antipyretic activities.

He concluded, “This study, therefore, supports its use in folk medicine both as an analgesic and anti-inflammatory agent and calls for further investigations to elucidate its mechanism of action.”

The anti-inflammatory effects were also supported by research in 2003 at the King Saud University, Saudi Arabia.


In 2005 at King Faisal University, Al-Ahsa, Saudi Arabia they studied the effects of black seed in conjunction with the antibiotic oxytetracycline (OXT) in pigeons

(an antibiotic commonly used to treat infections of the respiratory and urinary tracts.)

They concluded the addition of Black seed to the feed of pigeons could act as an immunoprotective agent when the chronic administration of antibiotics are considered.

Respiratory System

research3In most asian countries Black seed and it’s oil are commonly used for the treatment of asthma. Nigellone (a carbonyl polymer of thymoquinone) has proved to be an excellent prophylactic agent for both bronchial asthma and asthmatic bronchitis and has proved to be more effective in children than adults.


For cancer, you need to consult your physician.

Extracts of black seed have been patented to increase immune function during chemotherapy ( ).

One must note that this is not black seed as a whole. It is an extract.

It is worth giving this information to your physician, who may consider it as helpful.

Tumour Therapy

In 1997 at the Cancer Research Laboratory of Hilton Head Island, South Carolina, USA, it was proven that black seed oil had enormous success in tumor therapy without the negative side effects of common chemo-therapy.

They found that it increased the growth rate of bone marrow cells by a staggering 250% and it inhibited tumor growth by 50%. It stimulated immune cells and raised interferon production which protects cells from the cell destroying effect of viruses.

They confirmed the strong anti-bacterial and anti-mycotic, effects and that it has an effect in lowering the blood sugar level which is essential for the treatment of diabetes.

They concluded that a healthy immune system will detect and destroy cancer cells before cancer endangers the patient. They concluded that black cumin seed oil is an ideal candidate for use in cancer prevention and cure and that it has remarkable promises for clinical use.

Breast Cancer

In 2003 at the Jackson State University, USA, doctors who were interested in how Black seed had been used for such a long time for the treatment of so many acute ailments decided to study Black seed’s effectiveness in cancer prevention.

They exposed breast cancer cells to black seed extracts, the breast cancer cells were inactivated whereby they concluded that Black seed had promising results in the field of prevention and treatment of cancer.

Cancer of the Colon

Also in the same year doctors at Osaka City University Medical School in Japan, tested black seed for cancer of the colon where their studies displayed that the blessed seed inhibited the growth of cancer in the post-initiation stage.

In 2004 at the American University of Beirut, Lebanon, thymoquinone extracted from black seed was investigated to see if it triggers apoptotic cell death in human colorectal cancer cells. They concluded that thymoquinone has the potential for the treatment of colon cancer.

Anti-Tumor Effects

In 1991 at the Amala Research Center in Amala Nagar, Kerala (India) studies confirmed the use of black seed oil as an anti-tumor agent.

A second common type of cancer cells, Dalton’s lymphoma ascites (DLA) cells were also used. Mice which had received the EAC cells and black seed remained normal without any tumor formation, illustrating that the active principle was 100% effective in preventing EAC tumors development.

Anti-fungal; Anti-bacterial (candida)

research4In 2003 at the Aga Khan University Medical College, Karachi, Pakistan, the antifungal activity of Black seed was studied. Candida was inoculated in mice whereby the spleen liver and kidneys were infected.

Extracts from black seed were fed to the mice which caused a considerable inhibitory effect on the growth of the organism in all organs.

They concluded that the aqueous extract of black cumin seed exhibited an inhibitory effect against candidiasis, and that the traditional use of the plant in fungal infections was valid.

In 2005, in the Pakistan Journal of Medical Science, a paper was published where the antibacterial and antifungal effects of black seed were tested against standard and hospital strains of Candida albicans.nd Pseudomonas aeruginosin.

They were also investigated and compared with standard drugs, clotrimazole (an anti-fungal ointment used to topically treat vaginal candidiasis and candida of the scrotum and anus.), cloxacillin (a semisynthetic antibiotic in the same class as penicillin.) and gentamicin (an anti-biotic.) respectively.

They concluded that their results are in agreement with others who showed that black seed extracts produce antimicrobial activity against a broad range of microbes and especially on multiple antibiotic resistant bacteria.


In 2002 at the Gifu University, Japan, studies concluded that black seed may be of significant value to sufferers of type 2 Diabetes.

In1991 at Kuwait University, the mechanism of action was studied for black seed whereby they concluded that extracts may prove to be a useful therapeutic agent in the treatment of non-insulin dependent diabetes mellitus.

In 2003 three faculties in Van, Turkey came together and confirmed that black seed brought the lowered sugar level back to the control level in diabetic rabbits.

In 2004 at the Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey they tested the effects of Nigella sativa on diabetic rats.

They concluded that black seed treatment exerts a therapeutic protective effect in diabetes.


Haematological studies on black seed oil were made in 2001 at the Meiji Pharmaceutical University, Tokyo, Japan. They concluded that there was more potent activity than aspirin, well known as a remedy for thrombosis.

Cestodes (worms) in children

research5In 1991 at the University of Agriculture, Faisalabad, the anticestodal effect of Black seed was studied in children who had been naturally infected. They concluded that black seed contained active principles effective against these worms.

In 2007, Abdulelah and Zainal-Abidin investigated the anti-malarial activities of different extracts of nigella Sativa seeds against P. berghei. Results indicated strong biocidal effects against the parasite.

Black seed was found to have an anthelmintic activity against tapeworm comparable to that of piperazine. The pure essential oil showed activity against Monezia in sheep comparable to niclosamide.